ACTIVATION OF PURIFIED POLYPHENOL OXIDASE AND LACCASE OF CHICKPEA CV ICCV10 BY SODIUM DODECYL SULFATE

Abstract

The effect of sodium dodecyl sulfate (SDS) was studied on the purified polyphenol oxidases (PPO-1) and PPO-II/ laccase of chickpea cv ICCV 10 infected by Fusarium oxysporum f. sp. ciceri. Catachol and ABTS were found to be the the best substrates for PPOs and laccase enzymes respectively.PPO-I and PPO-II/laccase activities were increased by 10-40% at 10 µM of SDS. Benzoic acid was shown to be a competitive inhibitor in absence of SDS, where as in presence of SDS the type of inhibition was found to be noncompetitive. Therefore SDS is an activator of PPOs and lacaase enzymes that can probably change latent form of enzyme to active form, so increases the activities of PPOs.