IN VITRO SHOOT INDUCTION OF THREE GRAPE (VITISVINIFERA L.) VARIETIES USING NODAL AND AXILLARY EXPLANTS

Abstract

Nodal and axillary explants bearing two buds each from three varieties of grape (Vitisvinifera L.), viz., Crimson seedless, Bangalore Blue and Red Globe were micro propagated for induction of shoots on basal MS (Murashige and Skoog, 1962) medium. Nodal-bud cuttings performed well in all the three varieties: Crimson Seedless T2 V1 (71.43), followed by Bangalore Blue T2 V2 (27.27) and Red Globe T2 V3 (20.00), whereas, axillary-bud cuttings of just Crimson Seedless T1 V1 (15.29) survived, and, the other two varieties, Bangalore Blue T1 V2 (0.00) and Red Globe T1 V3 (0.00), failed to survive. Hence, from the present findings, it is concluded that nodal-bud cuttings from ‘Crimson Seedless’ grape variety are suitable for in vitro shoot induction on MS medium.