PREPARATION, CHARACTERIZATION AND ANTIBACTERIAL STUDIES OF SILVER NANOPARTICLES SYNTHESIZED BY SOLANUM NIGRUM SOLANUM NIGRUM SOLANUM NIGRUM LEAF EXTRACT

Abstract

Nanotechnology is anew emerging field where it finds applications in all the aspects of life sciences. In order to meet the increasing demands of therapeutic agents, new methods are being discovered for the eco-friendly synthesis of therapeutically important silver nanoparticles. Traditionally,silver nanoparticles are synthesized using chemical techniques, which involve often toxic chemicals. As an alternative to the conventional techniques, in the present study, an eco-friendly, cost effective method was suggested for biosynthesis of silver nanoparticles using the reduction of AgNO3 by the phytochemicals present in Solanum nigrum leaves.The colour change of the silver nitrate solution after addition of plant extract indicated the reduction of silver ions. The formation of peak around 420 nm in UV-Visible spectroscopy confirmed the presence of silver nanoparticles. The FESEM and HRTEM analysis revealed the spherical structure of the synthesized nanoparticles. The silver nanoparticles was further tested against human pathogens and was found to be highly toxic against tested bacteria.In conclusion, the silver nanoparticles synthesized using Solanum nigrum leaf extract showed better antibacterial activity against human pathogenic bacteria which can be employed for the treatment of bacterial infections