EFFECT OF DIFFERENT HYDROLYTIC ENZYMES ON GERMINATION OF INTER AND INTRA SPECIFIC COTTON HYBRIDS AND PARENTS

Abstract

Cotton (Gossypium spp.; Malvaceae family) is the world’s leading fiber crop and among the most important oilseed crops. It is the most essential textile fiber worldwide as it currently accounts for 90% of the commercially grown cotton. All the three hydrolytic enzymes such as amylase, protease and lipase are affecting the germination of cotton seeds. During germination of seeds, á-amylase and protease degrade starch granules and reserve proteins, respectively; thereby reducing the dietary bulk and improving the digestibility of starch and protein. Lipases are hydrolyzing triacylglycerols into fatty acids and glycerol. The present investigation revealed that hydrolytic enzyme (amylase, protease and lipase) activity of intraspecific hybrid (G.cot.Hy-12), interspecific hybrid (G.cot.Hy-102) and desi hybrid (G.cot.DH-9) are significantly increasing at 24, 48, and 72 hr in comparison of their parents. Among the different hybrids and parents intraspecific hybrid (G.cot.Hy-12) showed higher amylase (63.9 mg maltose/g tissue/min), protease (3.86 U/ml) and lipase (17.2 meq/min/g) activity respectively at 72 hr. Hydrolytic enzyme activity is decreases at 96 hr in all hybrids and their respective parents. Therefore, it can conclude that higher hydrolytic enzymes activity of all the hybrids shows higher germination percentage in comparison of their respective parents.