ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF SOIL INHABITING LACTOBACILLUS SPECIES MTCC 10093

Abstract

Lactobacillus species was readily isolated from wheat field of Chapra (Saran), Bihar, India, during a survey carried
out to study microbial flora of the region. This strain is Gram’s positive, rod shaped with size of 3-6µm and
capable of growing optimally in the media MRS (de Man Rogsa and sharpe). It utilizes a vast array of carbon
sources (viz starch, arabinose, glucose, etc). It grows at variable temperature and pH ranging from 12ºC to 42ºC
and 5.2 to 10.5 respectively. Further it can tolerate NaCl concentration up to 10 % w/v. Antibiotic sensitivity
study revealed that this is resistant to Streptomycin and Nalidixic acid while sensitive to Ciprofloxacin and
Azithromycin. It has been found that the isolated strain MTCC10093 is similar to Lactobacillus rhamnosus. This
strain is hitherto unknown and unreported from the area. This paper deals with the identification and
characterization of soil inhabiting Lactobacillus species. We report here, immense capability and adaptability of
this bacterium growing in diverse habitant and large spectrum of environment which makes it a successful
member among the probiotics bacteria