ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF SOIL INHABITING LACTOBACILLUS SPECIES MTCC 10093
Keywords:
Lactobacillus species, Probiotics, Antibiotic assay, Carbon sources, DNA profileAbstract
Lactobacillus species was readily isolated from wheat field of Chapra (Saran), Bihar, India, during a survey carried out to study microbial flora of the region. This strain is Gram’s positive, rod shaped with size of 3-6µm and capable of growing optimally in the media MRS (de Man Rogsa and sharpe). It utilizes a vast array of carbon sources (viz starch, arabinose, glucose, etc). It grows at variable temperature and pH ranging from 12ºC to 42ºC and 5.2 to 10.5 respectively. Further it can tolerate NaCl concentration up to 10 % w/v. Antibiotic sensitivity study revealed that this is resistant to Streptomycin and Nalidixic acid while sensitive to Ciprofloxacin and Azithromycin. It has been found that the isolated strain MTCC10093 is similar to Lactobacillus rhamnosus. This strain is hitherto unknown and unreported from the area. This paper deals with the identification and characterization of soil inhabiting Lactobacillus species. We report here, immense capability and adaptability of this bacterium growing in diverse habitant and large spectrum of environment which makes it a successful member among the probiotics bacteria