IN VITRO REGENERATION OF TION OF TION OF CAPE GOOSEBERRY (PHYSALIS PHYSALIS PERUVIANA L.) THROUGH NODAL SEGMENT

Abstract

A Protocol for in-vitro propagation of Cape gooseberry (Physalis peruviana L.) was standardized using nodal segments and internodal segments. Nodal segments (1-2cm) having atleast one axillary bud was cultured on Murashige and Skoog (MS) medium supplemented with BAP and IBA. Nodal explant was found better than internodal explants. The plant growth regulators BAP (2.5 mg/L) and IBA (0.05 mg/L) were judged to be best for shoot proliferation on the basis of sprouting percentage, number of shoots, shoots length and number of leaves. The regenerated shoots from nodal segments were excised aseptically and transferred to the rooting medium supplemented with IBA (0.05mg/L) and was found superior for days to root initiation, number of roots and root length. Addition of activated charcoal at 450 mg/L resulted in 100 per cent browning free culture.