CYTOPATHOLOGICAL ASSESSMENT OF UTERINE CELLS IN RATTUS NORVEGICUS DUE TO INDUCED SODIUM FLUORIDE

Abstract

Sexually matured female Wistar rat of weight ranging from 150-180g were used for the investigation. To
observe the cellular changes in uterus, experimental rats were intoxicated with sodium fluoride. Experiments
were carried out in four groups, in which first group was considered as normal group of animals, remaining
three as experimental which were intoxicated subcutaneously with 30mg NaF/kg bw/day, 45mg NaF/kg bw/
day and 75mg NaF/kg bw/day, respectively for continuously 15 days of duration. After completion of exposure
period, animals were sacrificed. Desired organ i.e. uterus was used to observe normal histology and
histopathologically cellular alterations in experimental animal groups by following standard microtechnique
procedure. The uterine sections in all groups were stained by Haematoxylin-Eosin staining. The normal cells
in uterus were compared with cells in intoxicated group of animals. Observed cellular alterations were
discussed with physiology of reproduction