Extraction and Evaluation of Leaf Protein Concentrate from Four Selected Grass Species.

Abstract

Leaf protein concentrate (LPC) represents a sustainable and economical source of plant protein with
growing importance in nutrition and feed research. The present study investigated the extraction and
protein potential of LPC obtained from four selected grass species: Cynodon dactylon, Dichanthium
annulatum, Eragrostis unioloides, and Setaria glauca. Fresh leaves were processed to obtain green
juice, followed by heat-induced coagulation and centrifugation for LPC recovery. The extracted LPC
was analysed for yield, moisture content, and protein concentration using standard analytical
procedures. Significant variations were observed among the grass species with respect to LPC yield
and protein content. Cynodon dactylon and Dichanthium annulatum showed comparatively higher
LPC recovery and protein levels than the other species studied. The results demonstrate that selected
grasses can serve as viable alternative sources of plant protein. This study highlights the potential of
utilizing underexploited grass resources for LPC production, contributing to sustainable protein
supplementation and improved feed and nutritional security.