Anti-bacterial and Anti-inflammatory Activity of Series of (5-{[(1H-1,2,3-triazol-5-yl) sulfanyl] methyl}-4H-1,2,4-Triazole-3-Thiols and (4-((1-phenyl-1H-1,2,3-Triazol-4-yl) methoxy) phenyl) diazenyl) benzo[d]Thiazoles

Abstract

Herein, we report the Anti-bacterial and Anti-inflammatory activity of Series of (5-{[(1H-1,2,3-triazol-5-yl)sulfanyl]methyl}-4H-1,2,4-Triazole-3-Thiols 1a-j and (4-((1-phenyl-1H-1,2,3-Triazol-4-yl)methoxy)phenyl)diazenyl)benzo[d]Thiazoles 2a-m shown in  Table-I to VIII . The antibacterial activity of the compounds prepared was evaluated by the filter paper disc technique of Vincent and Vincent.  The bacteria used in the present study were Escherichia coli, (Gram-negative) and Bacillus subtilis (Gram-positive).  The compounds were dissolved in acetone and tried at two different concentrations (250 and 500 mg/disc). The anti-inflammatory activity was evaluated by Carrageenan-induced rat paw edema method. All the experiments were carried out using male, Wistar rats (200-250g).  Test compound and Diclofenac sodium were suspended in 0.1% W/V carboxy methylene cellulose sodium (CMC) and administered intraperitoneally to animals. Carrageenan was diluted separately in normal saline and injected. Compounds 1e, 1f and 1j showed promising antibacterial activity. The most active compound of the series was 1e. Compounds 2g, 2j and 2m exhibited high inhibition, whereas 2b, 2c, 2e and 2h were moderately active. The most active compound of the series was 2g. Compounds 1c, 1e, 1f and 1j showed significant anti-inflammatory activity. Compounds 2c, 2e, 2g, 2j and 2m showed very good anti-inflammatory activity.

Keywords
Anaemia, adolescent girls, prevalence, occurrence, haemoglobin level, nutritional deficiency, school-based screening, and cross-sectional study.