EVALUATIONOFANTI-DIABETICACTIVITYOFFLAVONOID RICH FRACTION FROM METHANOLIC EXTRACT OF COCCINIAGRANDIS
Keywords:
Effectiveness, GNM3rdyear Students,, childhoodillnessIMNCI.Abstract
The present investigation aimed to evaluate the extraction efficiency, phytochemical profile, chromatographic
separation,toxicity,andantidiabeticpotentialofthemethanolicextractanditsisolatedfractionsfromCocciniagrandisfruit.The
methanolicextractexhibitedasignificantlyhigheryield(18.16%)comparedtothepetroleumetherextract (1.56%), indicating
superior extractionof polar bioactive constituents. Phytochemical screening revealed the presence of alkaloids, phenolics,
flavonoids, tannins,steroids, triterpenoids,andsaponins,whichwassupportedbyhightotalphenoliccontent(88.03mgGAE/g)
and total flavonoid content (71.31mgQE/g). Column chromatographyyielded eight fractions, amongwhichFractionF2
demonstratedthehighestflavonoidconcentration(138.37mgQE/g).AcutetoxicitystudiesconfirmedthatFractionF2wassafe
upto2000mg/kg. Invivoantidiabeticevaluationinalloxan-induceddiabeticratsshowedthatF2significantlyimprovedbody
weight, reducedfastingbloodglucose levels, andrestoredlipidprofiles inadose-dependentmanner.TreatmentwithF2also
normalized elevated biochemical markers such as SGOT, SGPT, creatinine, and urea, indicating hepatoprotective and
nephroprotective effects. These therapeutic responses areattributed to the flavonoid-richnatureofF2,whichmayenhance
insulinsensitivity,modulateglucosemetabolism,andattenuateoxidativestress.Thefindingsdemonstratethattheflavonoid-rich
FractionF2ofCocciniagrandisexhibitspotentantihyperglycemic,antihyperlipidemic,hepatoprotective, andnephroprotective
activities.ThestudyscientificallysupportsthetraditionaluseofCocciniagrandisindiabetesmanagementandhighlightsF2asa
promisingnaturaltherapeuticcandidateformetabolicdisorders.
