A new stability indicating UPLC-MS/MS methods for the simultaneous estimation of Abacavir, Dolutegravir and Lamivudine in pharmaceutical dosage forms

Abstract

Abacavir, Dolutegravir and Lamivudine are anti-viral drugs. A new stability indicating LC-ESI-MS method has been proposed and validated for the quantification of Abacavir, Dolutegravir and Lamivudine in pharmaceutical formulations as per ICH guidelines. WATERS, Quattro Premier XE Mass Spectrophotometer (MS/MS) with Software: Mass Lynx, Ver 4.1 and WATERS, Acquity UPLC with PDA detector was employed for the present study. A mixture of 0.2% Formic acid: Acetonitrile was used as mobile phase on gradient mode using Phenomenex Gemini C18, 50*4.6mm*5um with 0.5mL/min and run time of 3.5 min. A mixture of Acetonitrile: Water (80:20) was used as diluent. Linearity was observed over the concentration range 10.0-150ng/ml for Abacavir, Dolutegravir and Lamivudine by the proposed method and the methods are found to be simple, precise and accurate. Forced degradation studies were performed and the method is found to be selective and specific.