Effectiveness of Amikacin, Gentamicin and Tobramycin antibiotics against uropathogenic MDR Escherichia coli

Abstract

The emergence of multidrug-resistant (MDR) Escherichia coli poses a major global health concern, particularly in urinary tract infections (UTIs). The present study focuses on the isolation of E. coli strains from urine samples, their antimicrobial susceptibility profile, and molecular identification of MDR isolates using 16S rRNA gene sequencing. Urine samples were collected from patients with suspected UTIs and cultured on selective media for the isolation of E. coli. The isolates were subjected to antibiotic susceptibility testing against various classes of antibiotics using the disc diffusion method. MDR E. coli strains showing resistance to multiple antibiotics were further identified and confirmed by 16S rRNA gene sequencing. The Minimum inhibitory concentration (MIC) assays were performed to determine the efficacy of different concentrations of selected antibiotics. In the present investigation the E. coli isolates were isolated on MacConkey’s agar plate. The E. coli isolate SUC-1 showed resistance to antibiotics from class β-lactam, cephalosporin, sulphonamide, quinolones, macrolides, glycopeptides, polymyxin and synthetic antibiotics oxazolidinones. The MDR isolate SUC-1 was identified as Escherichia coli it was deposited in GenBank under Accession No. PP854585.  Among the tested antibiotics, aminoglycoside class drugs amikacin, gentamicin, and tobramycin showed significant inhibitory activity against MDR E. coli isolates, indicating their continued effectiveness. The findings suggest that aminoglycosides remain potent therapeutic options for treatment of MDR urinary infections.

KEYWORDS:

Escherichia coli, Urinary tract infection, Multidrug-resistant, Aminoglycosides