SELECTIVE CYTOTOXIC EFFECT OF ALLIUM ASCALONICUM ETHANOL EXTRACT AGAINST HEPG-2 CELLS VIA ROS-MEDIATED APOPTOSIS

Authors

  • Senthil kumar. K. S
  • Senthilkumar G P
  • R Lavanya
  • Linisha.N.M
  • Paranthaman

Keywords:

Allium ascalonicum, HepG-2 cells, Vero cells, MTT assay, DAPI staining, DCFDA staining, ROS, Apoptosis

Abstract

Cancer remains a leading global health concern, necessitating the search for effective and less toxic therapeutic agents

from natural sources. This study investigates the anticancer potential of ethanol extract of Allium ascalonicum (shallot)

against human hepatocellular carcinoma (HepG-2) cells, with Vero cells used as a normal control. The cytotoxic effects

were evaluated using the MTT assay, while DAPI and DCFDA staining were employed to detect apoptotic nuclear

changes and reactive oxygen species (ROS) generation, respectively. The extract demonstrated significant, dose-dependent

cytotoxicity in HepG-2 cells, with an IC50 value of 80 μg/mL, and showed minimal toxicity in Vero cells, indicating

selectivity. DAPI staining revealed characteristic apoptotic nuclear condensation, and DCFDA results confirmed enhanced

ROS production in treated cancer cells. These results suggest that Allium ascalonicum ethanol extract induces ROS

mediated apoptosis and may serve as a promising, selective anticancer agent for liver cancer therapy.

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Published

2025-09-24

How to Cite

Senthil kumar. K. S, Senthilkumar G P, R Lavanya, Linisha.N.M, & Paranthaman. (2025). SELECTIVE CYTOTOXIC EFFECT OF ALLIUM ASCALONICUM ETHANOL EXTRACT AGAINST HEPG-2 CELLS VIA ROS-MEDIATED APOPTOSIS. The Bioscan, 20(Special Issue-3), 980–986. Retrieved from https://thebioscan.com/index.php/pub/article/view/4139

Issue

Vol. 20 No. Special Issue-3 (2025): Volume 20 Special Issue 3 July (2025)

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.