EXPLOITING IN VITRO CULTURED VIGNA RADIATA FOR NATURAL ANTICANCER THERAPY AGAINST MCF-7 CELLS

Abstract

Vigna radiata (mung bean) is widely utilized in both traditional and modern medicine. In vitro propagation offers promising techniques for the conservation and enhancement of valuable medicinal plants. This study investigates the effect of various plant growth regulators on shoot and root induction using nodal segments cultured on Murashige and Skoog (MS) medium. Multiple shoot generation was observed within 7–8 days of incubation. Methanolic leaf extracts exhibited significant anticancer activity against MCF-7 breast cancer cells, with IC50 values of 91.074 µg/µl, indicating greater potency compared to acetone extracts (104.523 µg/µl). Apoptosis was confirmed using DAPI, propidium iodide staining, comet assay, and caspase-3 expression. This study highlights the therapeutic potential of V. radiata leaf extract as a natural anticancer agent.