Cardiospermum halicacabum derived MoO3 nanoparticles for Fluorescence sensing, DNA binding and Cytotoxicity Studies

Abstract

MoO3 nanoparticles were biosynthesised using Cardiospermum halicacabum leaf extract. Their structural and morphological properties were investigated through UV–visible DRS studies, photoluminescence, Fourier Transform Infra-Red spectroscopy, Energy-Dispersive X-ray analysis, X-Ray Diffraction, X-Ray Photoelectron Spectroscopy and High-Resolution Transmission Electron Microscopy. O2-→Mo6+ transition was confirmed by the peak observed at 353nm in UV-Visible spectrum and the band gap was estimated as 2.48 eV. Mo-O-Mo bond was confirmed by the bands observed at 842 cm-1 and 475 cm-1 in FTIR spectroscopy. EDX spectra showed characteristic peaks of oxygen and molybdenum. XPS showed binding energies of 232.4 and 235.6 eV, which correspond to spin-orbit splitting of Mo 3d5/2 and Mo 3d3/2 respectively, which confirmed that Mo is present in +6 state. The particle size was determined to be 69 nm using XRD analysis. Effective fluorescence sensing of Pb2+ was observed with a detection limit 4.13x10-8 M. ct-DNA binding constant was calculated as 3.09 (mg/mL)-1 and 2.32 (mg/mL)⁻¹ using UV-Vis and fluorescence studies respectively. Cytotoxicity studies using A549 Human Lung cancer cell line showed IC50 value of 249.5 μg/mL