Isolation and identification of different amino acids and other phytoconstituents through LCMS, GCMS analysis of Ananas comosus and evaluation of antifungal activity

Abstract

Aim-The project investigates the presence of different phytoconstituents and antifungal activities of protein concentrated part of Pineapple, scientifically known as Ananas comosus.
Objective-Pineapple scientifically known as Ananas comosus having different phytoconstituents unexplored and evaluation of antifungal activity have to be established.
Material and method-The pineapple juice have been extracted and lyophilized. Then ion exchange chromatography was performed to isolate different amino acid. Different salts were used for protein precipitation, and various solvents were used, including petroleum ether, n-hexane, ethanol, methanol, and aqueous media. Then this protein concentrated part was sent to TAAB Biostudy center, Kolkata, aqueous extract was send to IIB Kolkata for LCMS, n-hexane part was sent to GCMS to IICB, Kolkata,
The antifungal activity was performed with A. niger & C.albicans with ethanolis extract and two different protein concentration with Sabogaurd media.
Results-The amino acids glycine, alanine, phenylalanine, glutamic acid, histidine and arginine were found in isolated part of protein concentration. The LCMS study of aq extract of shows the presence of Gallic acid, Pyrocatechol, Hydroxytyrosol, Chlorogenic acid, Caffeic acid, Syringic acid etc. The GCMS study shows the presence of decane, 3,7-dimethyl-; benzene, 1,3-bis(1,1-dimethylethyl)-; dodecane, 4-methyl-; dodecane, 2,6,11-trimethyl-; heptadecane; 2,11-2,6,10-trimethyltridecane; nonadecane etc.
The antifungal activity was established for A. niger & C.albicans with ethanolis extract with ethanolic extract and protein concentrated part I.