GROWTH CURVE ANALYSIS OF BACTERIAL ISOLATES FROM FRESH COAL MINE SPOIL

Abstract

The research examines the growth curves of two Gram-negative bacteria, one isolated from fresh coal mine debris and the other from a temperature range of 35 to 50ºC. According to the results, both isolates exhibited a typical optimum development curve at 35ºC. Nonetheless, as the temperature increased, the two isolates had a distorted growth curve, with the specific growth rate and exponential phase both decreasing. The results show that both isolates can withstand a higher temperature regime, indicating that they are mesophilic. The research examines the growth curves of two Gram-negative bacteria, one isolated from fresh coal mine debris and the other from a temperature range of 35 to 50ºC. According to the results, both isolates exhibited a typical optimum development curve at 35ºC. Nonetheless, as the temperature increased, the two isolates had a distorted growth curve, with the specific growth rate and exponential phase both decreasing. The results show that both isolates can withstand a higher temperature regime, indicating that they are mesophilic.