Evaluation the micronucleus and comet assays in patients with hepatitis B in Samarra city/Iraq

Abstract

Hepatitis B virus (HBV) remains a significant global health concern, affecting millions of individuals worldwide. In this study, we aimed to explore these assays, their relevance in evaluating genetic damage in patients with HBV, and their potential implications for public health in the region. We employed the comet assay and MNi scoring to assess the induction of host DNA in the oral and renal epithelial cells of HBV-infected patients in comparison to healthy controls. The results revealed that the highest mean of mn were in HBV patients (4.11) compared to the healthy control group (0.26). The results showed that the BN in the HBV patient group was lower (0.38) than in the healthy controls group as (3.61). Furthermore, the KL was higher in the health controls (1.83) than that in the patients' group (1.78). Moreover, the TCWD was higher among HBV and healthy controls groups. Furthermore, there was no significant difference between the groups under study and healthy control groups at (p≤ 0.05). In conclusion, the results of this study revealed that HBV is a serious cause of liver disease in Samarra.