UV-B Radiation effects on Phytochemical composition of Senna auriculata (L.) Roxb: an HPTLC Study

Abstract

Objective: To investigate how UV-B radiation influences the phytochemical composition of Senna auriculata, utilizing High-Performance Thin-Layer Chromatography (HPTLC) to analyze and compare the ethanol extracts of untreated and UV-B-treated samples, thereby enhancing the understanding and standardization of the plant's medicinal properties. Method: The study employs High-Performance Thin-Layer Chromatography (HPTLC) to analyze and compare the ethanol extracts of untreated and UV-B-treated Senna auriculata to investigate the influence of UV-B radiation on its phytochemical composition. Results: The HPTLC analysis indicated that UV-B treatment results in qualitative and quantitative changes in the phytochemical composition of Senna auriculata, highlighting changes in the prominence and abundance of specific compounds. Conclusion: The study concludes that UV-B radiation induces significant qualitative and quantitative changes in the phytochemical composition of Senna auriculata, enhancing its medicinal properties and emphasizing the importance of HPTLC in standardizing and assuring the quality of medicinal plants.