MORPHOLOGICAL AND MOLECULAR VARIABILITY OF COLLETOTRICHUM TRUNCATUM (SCHW.) ANDRUS AND MOORE ISOLATES PATHOGENIC TO SOYBEAN IN INDIA

Abstract

Among the eleven isolates collected from six different states exhibited morphological variability with respect to colony colour varied from cottony white to dark gray, spore ranging between 20.10-23.00 x 3.2-4.50 to 21.10- 24.20 x 3.8-4.10 μm. The colony colour was dark gray in Bidar isolate of Karnataka, Ujjain isolate of Madhya Pradesh where as Bagalkot isolate of Karnataka and Kota isolate of Rajastan produced white colony colour. The rest of the other isolate produced distinct colony colour. There was no much difference between spore sizes of the different isolates collected. The molecular variability by using RAPD technique revealed genetic similarity coefficient of five isolates ranged from 37 to 66 per cent. The Maximum similarity of 66 per cent was observed between isolates Bagalkot (Bg) and Latur (La) followed by 58% between isolates Bagalkot (Bg), Ujjain (Uj). The similarity coefficient of 56 per cent was observed between isolates of Dharwad (Dh) and Ujjain (Uj) also in Umiam (Um) and Ujjain (Uj). Whereas, the least similarity of 37 per cent was observed between Dharwad (Dh) and Latur (La) isolates. The cluster analysis revealed sub cluster groups depending on same geographical locations with distinct variability.