AFLP MARKERS BASED DIVERSITY ANALYSIS AMONG WILD RELATIVES OF TOMATO COLLECTED FROM CHHATTISGARH REGIONS OF INDIA

Abstract

The cultivated Tomato (Solanum lycopersicum) is the second most consumed vegetable worldwide and a well
studied crop species in terms of genetics, genomics, and breeding. For the current study wild relatives consisted
of cherry and medium sized tomato fruits of 10 tomato genotypes were collected from different regions of
Chhattisgarh in order to find out the genetic diversity among these. AFLP analysis revealed that, a total of 2209
reliable polymorphic bands were observed in the 9 AFLP gels varied from 128 to 417, of which only 3 was
monomorphic across all 10 genotype. The average no of polymorphic bands present across genotype per primer
combination varied from 2.11- 5.48. All the primer combination used in this study has >0.800 PIC value and
ranged from 0.813 (ESA14 Vs MSA12) to 0.898 (ESA13 Vs MSA14). The Jaccard’s similarity coefficient for AFLP
data set varied from 0.13 to 0.60. The 10 genotype formed 2 major clusters at 13 % similarity level. The lowest
similarity (0.13) was between genotypes Raipur cherry and Janjgir long which were the most divergent while the
highest similarity (0.72) was found between the genotypes jashpur cherry and Janjgir cripping. This study
demonstrated that AFLP markers are effective for obtaining unique fingerprints of, and assessing genetic diversity
among, tomato wild relatives.