ELECTROPHORETIC PROTEIN PROFILING OF DIVERSE TOMATO GERMPLASM CONTAINING UNIQUE GENES AINING UNIQUE GENES

Abstract

Total soluble seed storage proteins profiles of 12 tomato genotypes, including breeding lines containing four specific genes, dg, hp-1, ogc and rin, two exotic breeding lines and varieties each, two indigenously bred varieties and one local cultivar were analyzed by SDS-PAGE on 12.5% gels. Polymorphism of about 34.41% was obtained from seed protein profiling and 85.45% of the pairwise comparisons of the 12 genotypes showed at least one different protein band. The seed protein weight of the tomato genotypes under study ranged between 98KD to <14.3KD and relative mobility ranged between 0.025 and 0.787. Polymorphism in protein banding appeared in all the molecular weight regions, but maximum polymorphism occurred in comparatively lower molecular weight region (20.1 to 29 KD) and hence, this region is useful in identification of tomato genotypes. Dendrogram based on electro phoretic data clustered the 12 genotypes in three major clusters and four sub-clusters under Cluster I. The clustering pattern depicted unique genotypic features of BCT-115(dg), BCT-119(hp), Berika(high lycopene) and BCT-111rin(rin). BCT-115 was the most diverse genotype with one unique band. SDS-Protein profiling emerged important for genetic analysis and it indicated a considerable amount of genetic diversity among the tomato germplasm employed in the present investigation.