AMELIORATIVE EFFECTS OF VITAMIN-C AGAINST BISPHENOL-A TOXICITY IN LIVER OF CIRRHINUS MRIGALA (HAM.)

Abstract

180 fishes Cirrhinus mrigala 60±5 g were divided into three groups of 50 each. Group 1st received fish diet only and served as control, while group 2nd received Bisphenol-A (2mg/L) and group 3rd received vitamin-C (50 mg/L) along with Bisphenol-A (2mg/L) for 30 and 60 days and the enzymological (GOT, GPT, ACP and ALP), total protein, creatinine levels, histochemical and histopathological studies were estimated in their liver of Cirrhinus mrigala. Bisphenol-A significantly elevated the hepatic GOT, GPT, ACP and ALP enzyme activities levels after 30 and 60 days as compared with control group. Apart from this, Bisphenol-A lowered hepatic protein levels insignificantly, whereas its insignificantly elevated hepatic creatinine levels in Cirrhinus mrigala after 30 and 60 days in comparison to control group. In connection to this, BPA induced histopathological changes in liver after 30 and 60 days showed hypertrophied, as well as atrophied changes in hepatic cells, characterized by pycknotic nuclei with less amount of cytoplasmic materials. In histochemical studies i.e. bromophenol and silver nitrate reactions also denotes that protein and ascorbic contents respectively were lowered in later part of the experi- ment. However, animals supplementation with vitamin-C along with Bisphenol-A showed recovery in hepatic cells structures, hepatic enzyme activities, protein and creatinine contents in comparison to Bisphenol-A group. Above finding may suggests that vitamin-C ameliorated toxic effects of Bisphenol-A in Cirrhinus mrigala