A COMPARATIVE STUDY OF CRYO AND PARAFFIN SECTIONS IN LOCALIZING GASTRIN CELLS (G-CELLS) IN THE STOMACH OF RANA CYANOPHLYCTIS (SCHNEIDER) USING IMMUNOCYTOCHEMICAL TECHNIQUE

Abstract

The objective of the present study was to identify the gastrin immunoreactive (G IR) cells in the stomach of Rana cyanophlyctis (Schneider). For the present study both cryosections and paraffin sections of the stomach were used for immunocytochemistry. Both open and closed types of cells have been observed. The occurrence of G IR cells was found to be amidst gastric mucosal epithelium, around gastric glands with prominent secretory granules and also in muscularis region. Such presence was assumed to be their strategic position for secretion. The cryosections were more convenient for study in comparison to paraffin sections. Counter staining for paraffin sections is suggested.