ANGIOSTATIC PROPERTY OF CHITOSAN ACETATE IN THE CHICK CHORIOALLANTOIC MEMBRANE (CAM), IN OVO

Abstract

Chitin is the most ubiquitous natural polysaccharide after cellulose on earth. A wide variety of medicinal applications for chitin and chitosan (deacylated chitin) have been reported. In the present investigation the salt of chitosan-chitosan acetate was used to study its effect on angiogenesis using Chorioallantoic Membrane (CAM) assay in ovo. The solution of chitosan acetate was tested by administration at 48, 72 and 96 hours of incubation in chick eggs. Morphometric and microscopic examination of CAM indicated inhibition of neovasculature. The inhibition was reported in number and growth of secondary and tertiary blood vessels. There is 16.36%, 20.29% and 30.52% inhibition in number of secondary and 9.42%, 10.99% and 20.20% inhibition in number of tertiary blood vessels after 48hrs, 72hrs, and 96hrs of chitosan acetate treatment. It indicates that chitosan acetate affects endothelial growth factors having angiostatic property and may be used to control tumor growth.