PRODUCTION OF HIGH QUALITY EMBRYOGENIC CALLUS OF RICE

Abstract

The study was undertaken to standardize an efficient and effective protocol for callus Induction, subsequent growth and regeneration in rice variety Swarna Sub1.MS, B5 and N&N media were used for callus induction.Overall,MS medium was found better for callus induction as compared to N&N medium. Callus induction percentage was highest in SS29 (80.00) followed by SS4 which gave 76.67%, SS32 and SS44 gave 66.67%.  The growth Regulator 2, 4-D with varying concentrations were tested for callus induction. Production of embryogenic calli increased as the concentration of 2, 4-D was increased from 0.5 mg/L to 1.5 mg/L.