FACTORS INFLUENCING IN VITRO GROWTH AND SHOOT MULTIPLICATION OF POMEGRANATE

Abstract

Maximum proliferation of shoot (78.25 %), number of shoot (3.75) per explants and shoot length (3.06 cm) were obtained on MS medium supplemented with 1. 0 m g/L B A P + 1. 0 mg/L kinetin with 40 mg/L adenine sulphate in medium. Significantly higher number of shoots (4.5) and maximum length (3.38 cm) of shoot were recorded in 3 per cent sucrose. Maximum multiplication of shoot and length of shoot was observed at 3 per cent sucrose. Maximum number of shoots/explants (4.80) and shoot length (3.50 cm) was obtained under high (3000 lux) light intensity. Higher number of shoots per explant (4.25) and maximum length of shoot (3.70 cm) were recorded with pH 5.8. Multiplication and growth of shoots were significantly influenced by the different level adenine sulphate, sucrose, light intensity and media pH.