Identification of Immunity-Inducing Biological Compounds from Coelomic Fluid of Eudrilus eugeniae

Abstract

The coelomic fluid of Annelids exerts numerous biological activities
that are involved in effective defense mechanisms against invaders. The
coelomic fluid of the earthworm contains more than 40 proteins and exhibits
several biological activities. About 20μl of Pseudomonas aeroginosa culture
inoculated broth were injected into earthworm and disturbed at 24 hours to
obtain the coelomic fluid. The uninoculated broth alone served as the control.
P. aeroginosa injected coelomic fluid exerted higher antibacterial, proteolytic
and erythrolytic activity along with a newly expressed protein in HPLC. This
concludes that when the appearance of newly expressed protein in the body
cavity of earthworm automatically kills various foreign intruders.