SCREENING AND ISOLATION OF INTRACELLULAR α- GALACTOSIDASE PRODUCING MICROORGANISMS FROM SUGARCANE WASTE SOIL

E. SIRISHA; A. NAVEEN; V. BHARGHAV; M. LAKSHMI NARASU

SCREENING AND ISOLATION OF INTRACELLULAR α- GALACTOSIDASE PRODUCING MICROORGANISMS FROM SUGARCANE WASTE SOIL

Authors

E. SIRISHA
A. NAVEEN
V. BHARGHAV
M. LAKSHMI NARASU

Keywords:

Acinetobacter, Intracellular, Raffinose, α-Galactosidase

Abstract

Intracellular α-galactosidase (EC 3.2.1.22) producing bacteria were isolated from soil. The isolates were
grown on the growth media with sucrose as inducer. The isolate showing maximum activity was identified by
following Bergey’s manual. Different media parameters were optimized for enhanced enzyme production. α-
galactosidase activity was optimal at 36ºC and pH 7.0 and when raffinose and tryptone were used as carbon
and nitrogen source.

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Published

2010-12-13

How to Cite

E. SIRISHA, A. NAVEEN, V. BHARGHAV, & M. LAKSHMI NARASU. (2010). SCREENING AND ISOLATION OF INTRACELLULAR α- GALACTOSIDASE PRODUCING MICROORGANISMS FROM SUGARCANE WASTE SOIL. The Bioscan, 5(4), 579–582. Retrieved from https://thebioscan.com/index.php/pub/article/view/534