IN VITRO GERMINATION AND CALLUS INDUCTION FROM SEEDS OF CARTHAMUS TINCTORIUS L.

Abstract

The seeds of the plant Carthamus tinctorius commonly known as Safflower were cultured on a plain MS media for germination. The regenerated different plant parts were cultured in the Murashige and Skoog medium supplemented with different concentrations of phytohormones. The concentrations of different phytohormones taken of 2, 4-D, IBA, IAA and KN either in isolation or combination with each other. MS media was also prepared in combination with coconut milk (20% and 80% v/v). The best callus was induced in different concentrations of 2, 4-D from different parts regenerated from seeds like root, lower hypocotyls, upper hypocotyls, leaf. Callus induction was also found in coconut milk supplemented media and in combination of different Phytohormones like 2, 4-D + IAA, 2, 4-D + IBA, IAA + KN, 2, 4-D + KN. Best result was observed in 2, 4-D in 3.0 ppm – 5.0 ppm.