QUALITATIVEANDQUANTITATIVESTUDYOFPHYTO-COMPOUND PRESENTINROOTSOFCLITORIATERNATEAUSING SPECTROSCOPYANDCHROMATOGRAPHICTECHNIQUES

Abstract

The present studywas undertaken to evaluate the phytochemical profile, thin-layer chromatographic (TLC)
characteristics,andtotalphenolicandflavonoidcontentofrootextractsofClitoriaternatea.Successiveextraction
was carriedout usingsolventsof increasingpolarity (hexane, chloroform, ethyl acetate, ethanol, anddistilled
water).Theextractivevalues indicatedmaximumyieldwithaqueous (7.25%w/w) andethanolic(6.67%w/w)
solvents, suggesting a predominance of polar constituents. Preliminaryphytochemical screening revealed the
presence of flavonoids, phenols, proteins, carbohydrates, saponins, and diterpenes indifferent extracts, with
flavonoidsbeingconsistentlydetectedinethylacetate,ethanol,andaqueousextracts.TLCanalysisconfirmedthe
presenceofmultipleflavonoidcomponents,withethanolandethylacetateextractsshowingthehighestdiversity
and quercetin-like compounds (Rf ≈ 0.63). Quantitative estimation demonstrated that the ethanolic extract
contained thehighest phenolic (1.04mg/100mg) and flavonoid (1.65mg/100mg) content, followedby the
aqueousextract(flavonoids:1.41mg/100mg).Thesefindingssuggest thatethanolisthemostefficientsolventfor
extractingphenolicandflavonoidconstituentsfromClitoriaternatearoots,highlightingitspotential for further
pharmacologicalandtherapeuticinvestigations