PHYTOCHEMICAL SCREENING AND STANDARDIZATION OF VARUNA TAILA THROUGH HPTLC (HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY)

Abstract

Background- Varuna taila, is an herbal ayurvedic medicine mentioned in Bhaishjya ratnavali and Adarsha nighantu, includes the leaves of Crateva nurvala (Varuna), processed with Crateva nurvala (Varuna) juice (Swarasa) & Tila Taila and used as a remedy for urinary tract infections (UTIs), Striae Gravidarum. This formulation is prepared to be given in Striae gravidarum (Kikkisa) in Pregnancy. There are several methods available today for managing stretch marks, which could be expensive and painful. Ever more important is the knowledge imparted to a worried mother that her child will be fine and that the management will be without complication and low price. Therefore, there is a need to develop standardized Ayurvedic formulation in new dosage form that is safe and effective for pregnant women.
Aim- The primary objective of this research was to conduct physicochemical standardization and high performance thin layer chromatography (HPTLC) analysis of Varuna Taila.
Results- The Pharmaceutical assessment of the oil revealed that it exhibited a yellowish green color. Loss on Drying is 0.09, Total Ash value is 0.596, pH is of 4.3, Refractive index is 1.4500, Specific gravity is 1.0901, Viscosity is 35.2960, Acid value is 0.8976, Rancidity test is Negative, Saponification value is 210 & Iodine value is 97. HPTLC analysis of Varuna Taila was performed using the CAMAG Linomat 5 HPTLC system. The outcomes were presented as chromatograms, with peaks depicted after scanning at wavelengths of 254 nm, 366 nm, and 540 nm. The drug's phytochemical profile was assessed and illustrated through tables that displayed various parameters including the total count of peaks, peak heights, peak areas, percentage area, and Rf values.
Conclusion- The present study focused on the phytochemical characterization of Varuna taila. The HPTLC profile generated can serve as a preliminary tool for ascertaining the authenticity and standardization of the formulation. While Varuna taila has been traditionally indicated for conditions such as striae gravidarum.