EFFECTIVENESS OF PGR FOR IN VITRO CLONAL PROPAGATION OF LINDERNIA DUBIA (L.) PENNELL

Abstract

The study focused on standardizing the in vitro organogenesis process for Lindernia dubia (L.) Pennell, a less-concerned emergent plant of the Linderniaceae family, utilizing a micropropagation technique in hormonal MS medium. Two concentrations of MS medium were tested: half-strength and full-strength, with the half-strength medium showing superior results in regeneration. Various concentrations of BAP, KIN, and GA3 were employed for shoot induction, while NAA and IAA were used for root induction. After a month post-inoculation, developed shootlets were evaluated, and statistical analyses, including ANOVA and Tukey's Multiple Comparative Analysis, were performed. Regeneration rates fluctuated from 70% to 100%, with half-strength medium outperforming full-strength in all parameters. The peak performance was noted with 8 μM BAP, yielding 11.9 cm shoot lengths and 16.2 roots averaging 10.5 cm in length in the NAA rooting medium. The full-strength medium averaged 46 shoots and 5.7 roots. In combinations of auxins and cytokinins, the 4+2 μM BAP + KIN combination produced 64.9 shoots, while KIN + GA3 yielded 63.9 shoots at 8+4 μM. Auxin-rich media (NAA + IAA) facilitated root regeneration across all combinations, recording a maximum of 2.4 roots with an average length of 1.2 cm in the BAP + KIN combination. The conclusion drawn emphasizes BAP and NAA as optimal hormones for regenerating L. dubia in half-strength medium, with BAP + KIN and KIN + GA3 being effective combinations. This established micropropagation procedure aims to enhance the population of this plant to support sustainable conservation and provide essential raw materials for future medicinal research benefiting society.