TAXONOMY AND PHYLOGENY OF Limnodrilus hoffmeisteri, Claparède, 1862 BELONGING TO FAMILY TUBIFICIDAE d’ Udekem, FROM DIFFERENT WATERBODIES OF JAHARKHAND BASED ON DNA BARCODING

Authors

  • Rakesh Ranjan
  • Manoj Kumar
  • Rohit Srivastava
  • Bharti Singh Raipat
  • Manoranjan Prasad Sinha

DOI:

https://doi.org/10.63001/tbs.2025.v20.i01.pp239-245

Keywords:

Limnodrilus hoffmeisteri, morphology, DNA barcoding, distance matrix, phylogenetic tree, Jharkhand

Abstract

Aquatic Oligochaetes were collected from different habitats located in different districts of Jharkhand. Specimens of Limnodrilus hoffmeisteri, Claparède, 1862 was examined in details for ascertaining its taxonomy and phylogeny. Apart from the morphological details, the molecular study based on DNA barcoding was done for the first time for the specimen from this region. Based on the BLASTn results, the specimen was identified as Limnodrilus hoffmeisteri, after which the 18s rRNA nucleotide sequence was submitted to DNA databank of Japan and accession ID LC843736 was obtained. The sequence was used to prepare the distance matrix and phylogenetic tree using MEGA X software. The matrix score ranged from a minimum of 0.00000 to a maximum of 0.00329. The Ranchi strain of Limnodrilus hoffmeisteri (LC843736.1) differed from that of the other 10 (already available in gen bank with a matrix value of 0.00329 in each case). This indicates that the strain of Limnodrilus hoffmeisteri being reported by us form this region may be from an entirely different genetic pool as compared to the other strains selected in this study. From the phylogenetic tree it can be easily seen that the Ranchi strain lies singly in a separate clade from the selected 10 strains which fall in another clade.

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Published

2025-01-29

How to Cite

Rakesh Ranjan, Manoj Kumar, Rohit Srivastava, Bharti Singh Raipat, & Manoranjan Prasad Sinha. (2025). TAXONOMY AND PHYLOGENY OF Limnodrilus hoffmeisteri, Claparède, 1862 BELONGING TO FAMILY TUBIFICIDAE d’ Udekem, FROM DIFFERENT WATERBODIES OF JAHARKHAND BASED ON DNA BARCODING. The Bioscan, 20(1), 239–245. https://doi.org/10.63001/tbs.2025.v20.i01.pp239-245