A PSYCHROTOLERANT STRAIN KLUYVERA INTERMEDIA SOLUBILIZES INORGANIC PHOSPHATE AT DIFFERENT CARBON AND NITROGEN SOURCE

Abstract

A Kluyvera intermedium is a Gram negative psychrotolerant bacterium isolated from a high altitude rhizospheric soil from the Uttarakhand Himalayas where all over year temperature remains very low. This strain exhibited plant growth promoting traits both at 10°C and 30°C, under in-vitro conditions. The expression of plant growth promoting (PGP) traits like Indole acetic acid production by the bacterium was highest at 30°C, with a proportionate reduction in PGP activity at lower temperatures. Determination of phosphate solubilization by the bacterium at two incubation temperatures (10°C and 30°C) revealed a steady increase in the soluble phosphorus levels across the incubation temperatures, coupled with a concomitant drop in the pH levels of the culture supernatant, till the 10^th day of incubation. The Uttrakhand soil has acidic in nature and the phosphorus applied to the soil get fixed and become unavailable to the plant. So, to provide phosphorous easily to the plant, this strain has been chosen because this strain have the capacity to solubilize unavailable phosphorus. The phosphorus solubilising ability has been increased while a different carbon and nitrogen source has been used. This strain show better result when glucose is taken as carbon source and ammonium sulphate as nitrogen source.