ISSR BASED GENOTYPIC DIFFERENTIATION OF GRAPE (VITIS VINIFERA L.)

Abstract

In order to study the genetic variability of four grape cultivars (Nanasaheb purple, Sonaka, Thompson seedless
and Ganesh), 10 ISSR primers were screened of which seven were found polymorphic. These polymorphic
primers produced a sum total of 86 bands of which 56 were polymorphic. The grape cultivars grouped into two
major clusters at 51 per cent similarity. The first cluster had only Nanasaheb purple whereas; the second cluster
contained Sonaka, Thompson seedless and Ganesh cultivars. Thompson seedless and Ganesh in the second
cluster showed a similarity coefficient of 0.63. Clustering was strongly supported by high bootstrap values.
Resolving power of the ISSR primers ranged between 3 (UBC 850) and 10 (UBC 810), PIC value from 0.78 (UBC
850) to 0.88 (UBC 811, UBC 815 and UBC 834), and Marker indices (MI) from 3.89 (UBC 850) to 8.80 (UBC
815 and UBC 834) with a mean value of 6.14, 0.85, and 6.88, respectively. The results revealed that ISSR could
be a better tool for evaluation of genetic diversity among the grape cultivars.