PROTEIN AND ISOZYME PATTERNS OF RHIZOCTONIA BATATICOLA ISOLATES CAUSING CHICKPEA ROOT ROT

Abstract

Marked variations among different isolates of Rhizoctonia bataticola collected from various locations of Maharashtra were studied in respect of protein and isozyme analysis on the basis of pathogenecity. Protein content in different isolates of R. bataticola ranged from 0.756 mg to 1.021 mg/100 mg mycelium and maximum protein content was observed in R26 highly pathogenic isolate. The peroxidase activities was ranged between 39.35 to 69.79 U min-1 mg-1 protein and polyphenol oxidase activities from 0.17 to 1.10 U min-1 mg-1 protein among different isolates of R. bataticola. The maximum PPO activities was recorded in highly pathogenic isolate R34 of Aurangabad. Moreover, the maximum esterase activities among R. bataticola isolates was observed in moderately pathogenic R10 isolate of Buldhana district (9.92 μm min-1 mg-1 protein). Electrophoretic studies by using SDS-PAGE revealed that, seven soluble proteins bands between molecular weights 58.88 to 102.3 KDa and 0.05 to 0.25 Rf values were detected among the isolates of R.bataticola, however, weakly pathogenic isolates (R14, R19 and R24) are identical for expressing a common protein band of 0.15 Rf. The only common band of esterase (0.045 Rf) was noticed in slightly pathogenic isolates (R8, R41 and R47) which was absent in other isolates and the presence of common PPO band 0.024 Rf in all isolates of highly pathogenic isolates (R26, R34 and R54), indicates variability among isolates. Peroxidase as molecular marker also differentiates variability but existence was in traces i.e. maximum one band in most of the isolates, except isolates R41 (0.034 and 0.103 Rf) and R34 (0.034 and 0.092) which forms two bands, while no band was noticed in R54 isolate from Satara district.