CALLUS INDUCTION IN A MEDICINAL PLANT MURRAYA KOENIGII SPRENG

Abstract

Callus proliferation occured on leaf and stem. Explant cultured on Murshige and Skoog’s medium (MS) supplemented with 2, 4-D, IBA, NAA and BAP separately or in combination. In the beginning green callus was observed which turned into light yellow and was followed by formation of brown callus. Sometime light green callus also observed. Development of callus was observed from different concentration of auxin or cytokinin alone or in combination callus from shoot tips and nodal was that 2, 4-D (2.5mgL-1) and IBA (0.5mgL-1)