ANALYSIS OF RELATED SUBSTANCES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY [HPLC] METHOD FOR VALACYCLOVIR HYDROCHLORIDE

Abstract

Valacyclovir hydrochloride is a purine nucleoside analogue derived from Acyclovir and is used in the treatment of Herpes simplex and Herpes zoster infections. Valacyclovir hydrochloride is used to treat certain infections like Genital Herpes and Herpes cold sores on the face and lips. In the present study, it was aimed to perform qualitative analysis of related substances by High performance liquid chromatography [HPLC] for Valacyclovir hydrochloride, to obtain auto scaled chromatogram for the standard and impurity blends of Valacyclovir hydrochloride. The flow rate of the compound was maintained at 1.0micro/minute. The column used for Valacyclovir hydrochloride analysis is Inertsil ZODS 3V, 250X4.6mm ID, 5 micro meter particle size. Column temperature was maintained to 40°C.Different peaks were observed at different time intervals. Peak purity was checked for principle compounds and also for impurities. Valacyclovir was as effective and well tolerated in the treatment of recurrent genital herpes simplex virus infection as 5-times-daily acyclovir. Valacyclovir is well tolerated first line therapy with an established place in the treatment of immunocompetent patients with Herpes zoster.