PARTIAL PURIFICATION AND DETECTION OF HYALURONAN OLIGO BINDING PROTEIN IN HUMAN BREAST CANCER TISSUE

Abstract

Hyaluronan is a versatile molecule that can form variety of periodic multimolecular structures through its
interaction with specific HA binding proteins. There is increasing evidence showing, HA oligosaccharides
expression was associated with malignant progression. HA-oligosaccharides have diverse and complex biological
functions both In vivo and In vitro and are shown to enhance or prevent tumor growth, depending on the size of
the fragment through interaction with its receptors. We produced HA 8-20 mers size fragments by enzymatic
digestion of HA polymer and characterized by fluorescent assisted carbohydrate electrophoresis. Expression of
hyaluronic acid binding proteins for HA polymer and HA oligosaccharides were detected by western blot
analysis, which suggested that HA polymer has detected multiple receptors, while HA fragments identified
mainly 80 and 66 kDa receptors with enhanced expression in malignant breast tumor, which suggested the
increased specificity of HA oligosaccharides to its receptors.