BIOCHEMICAL CHANGES IN THE PROFILE OF CARBOHYDRATE, PROTEIN AND TRIGLYCERIDES IN THE BLOOD OF CLARIAS BATRACHUS DUE TOSUBLETHAL TREATMENT WITH INSECTICIDE ROGOR.

Abstract

In present study, the toxicity of insecticide rogor on biochemical parameters (carbohydrate,protein and triglycerides)
of fresh water fish Clariasbatrachus were estimated.The LC 50 ( 96 hours) of rogor for fish have been calculated as
4.5μl/L . Two sub lethal concentration selected in the experimental protocol were 1μl/L and 2.5μl/L of Rogor and
accordingly stock solutions of rogor were prepared using distilled water.Fishes were sacrificed after 5 th ,10 th ,15 th days
of exposure.The total protein count shows great variations ,at 1μl/L , the total protein first decreased on 5 th day by
39.7% but increased on 10 th and 15 th day although it was 15.8% lower than the protein count of controlled fish.
However, on 2.5μl/L the serum protein of treated fish increased by 10.5% on 5 th day of the treatment and on 15 th it
showed an increase of 39.5% as compared to the controlled fish. The glucose level also increased in comparison
to the controlled fish. On 1μl/L , the serum glucose increased by 15% on 5 th day of the treatment but on 10 th and
15 th day it decreased by 3.9% and 15% respectively. Whereas on 2.5μl/L ,the serum glucose increased by 18% on
5 th day,33% on 10 th day and 29% on 15 th day of the treatment. Triglycerides shows reduction in both doses , at 1μl/
L triglyceride reduced by 1.7%, 16.7% and 19.5% respectively, on 5 th day ,10 th day and 15 th day . On 2.5μl/L the
decline in the triglyceride of treated fishes in comparison to control fish, on 5 th , 10 th and 15 th day are 30.6%, 39.6%
and 41.6% respectively. This shows that rogor exposure hampers the nutritional value of these commercially
important fishes.